blunt tipped forceps  (Fine Science Tools)

Journal: STAR Protocols

Article Title: Para-uterine imaging protocol: Stabilizing living mouse embryos within the maternal abdomen for in vivo imaging and visually guided physiology

doi: 10.1016/j.xpro.2026.104446

Figure Lengend Snippet: Preparation for para-uterine imaging (A) Preparatory Steps 1 - 3: Setup prior to the start of surgery. 1: Mouse temperature controller, with associated heating pad and temperature probe. 2: Shaver. 3: UV-protective glasses. 4: Norland 65 UV-curing glue. 5: Eye lubricant. 6: Cover glasses. 7: Plastic pipette. 8: Retractors (x4). 9: Embryo holder. 10: Headbar. 11: Fine scissors. 12: Spring scissors. 13: Blunt-tipped forceps (x2). 14: Ring-tipped forceps. 15: Fine-tipped forceps (x2). 16: Binocular dissection scope. 17: HBSS. 18: Alligator clamp. 19: Underpad. More details in A. (B – L) Stepwise progression of surgery. (B) Preparatory Steps 8, 9 and Steps 1a, 1b: Placement of the dam in a supine position, followed by sterilization, removal of fur from the abdomen, and cutting through the skin. (C) Steps 1a, 1b: Cutting through the muscular fascia. (D) Step 1c, 3: Using retractors to open the abdominal cavity, exposing the uterine horns, and opening up the uterus. (E) Steps 4: Selecting an embryo and then opening up the amniotic sac to expose the embryo. Preparing an alligator clamp to stabilize the embryo holder. (F) Step 7: Placing the embryo gently in the embryo holder. (G) Step 9: Transferring the embryo holder to the alligator clamp. (H) Step 11: Adding agar to the embryo holder. (I) Step 12: Adhering the headbar to the embryo holder. (J) Step 13: Transferring the embryo holder to the external supports. (K) Steps 15, 16: Removing the skin (and bone) from the head of the embryo. Inset: Exposed embryonic brain (process detailed in ). (L) Step 19: Cover glass added to the embryo. Inset: Embryonic brain covered by cover glass, with the space filled with cortex buffer (process detailed in ).

Article Snippet: Blunt-tipped forceps , Fine Science Tools , 11652–10.

Techniques: Imaging, Transferring, Dissection

Journal: STAR Protocols

Article Title: Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination

doi: 10.1016/j.xpro.2023.102164

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Article Snippet: Dumont Forceps - Micro-Blunted Tips , Fine Science Tools , 11253-20.

Techniques: Marker, Recombinant, Membrane, Blocking Assay, Derivative Assay, Selection, Staining, Software, Cell Culture, Sterility, Suction Filtration, Pore Size

Journal: Journal of visualized experiments : JoVE

Article Title: Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

doi: 10.3791/63682

Figure Lengend Snippet: Materials

Article Snippet: Name Company Catalog Number Comments 1⁄2” Scotch Permanent Double-Sided tape Scotch 3M 665 0.1–10 μL uTIP pipette tip Biotix M-0011-9FC 22 × 22 mm No. 0 thickness coverslips Thomas Scientific 1207Z28 24 × 60 mm coverslip Corning 2980-246 3M VetBond 3M 1469SB Called “adhesive glue” in protocol Anti-FasIII primary Mouse IgG2a Developmental Studies Hybridoma Bank 7G10 Calcium Chloride Fisher Chemical c79-500 Cy3-conjugated AffiniPure Goat Anti-Mouse IgG2a Jackson ImmunoResearch 115-165-206 DAPI Sigma Aldrich D9542-1mg Dumont #5 Fine Tip Forceps Fine Science Tools No. 11254-20 Dumont #5 Fine Tip Forceps: Blunt Fine Science Tools No. 11254-20 Heavily used and unsharpened forceps, or dulled with a whetstone Fisherbrand Double frosted microscope slides Fisher Scientific 22-034-486 Fluorescent Light Source Lumencor Celesta 90-10512 Fly Stock: EGFP-tagged Histone H2A Bloomington Drosophila Stock Center 24163 Fly Vial Foam Plugs “Flugs” Genesee Scientific 49-101 Humidified Chamber: Foil-wrapped small container lined with filter paper saturated with water Cole-Parmer 759075D A great humidified chamber can be made from the styrofoam box containing Cole-Parmer cuvettes, 759075D, filled with 50 ml water.

Techniques: Transferring, Adhesive, Microscopy, Software, Laser Capture Microdissection, Plasmid Preparation

Journal: STAR Protocols

Article Title: Pipeline for 2-photon all-optical physiology in mouse: From viral titration and optical window implantation to binarization of calcium transients

doi: 10.1016/j.xpro.2021.101010

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Article Snippet: Dumont 590 Forceps - Micro-Blunted, Standard Tips/Angled 90°/Inox/11cm , Fine Science Tools , REF.: 11253-29.

Techniques: Virus, Plasmid Preparation, Recombinant, Saline, Cream, Electron Microscopy, Software, Sterility, Marker, Microscopy